Laboratory Excellence Standards: This guide provides comprehensive protocols for handling research-grade peptide compounds. Following these procedures ensures optimal stability, prevents contamination, and maintains the integrity required for reproducible research results.
🧊 Storage Guidelines
Critical: Proper storage is essential for maintaining compound stability and research validity. Temperature fluctuations and moisture exposure can degrade peptide integrity.
Pre-Reconstitution Storage (Lyophilized Powder)
| Duration | Temperature | Conditions | Notes |
|---|---|---|---|
| Short-term (up to 30 days) | Room Temperature (20-25°C) | Dark, dry location | Original sealed vial |
| Medium-term (1-6 months) | Refrigerated (2-8°C) | Moisture-proof container | Desiccant packets recommended |
| Long-term (6+ months) | Frozen (-20°C or below) | Waterproof, airtight container | Avoid freeze-thaw cycles |
Post-Reconstitution Storage (Solution)
Standard Protocol: Always refrigerate reconstituted solutions at 2-8°C. Use within 30 days for optimal stability.
- Store in original rubber-stoppered vial
- Maintain refrigeration at 2-8°C (36-46°F)
- Keep away from light sources
- Label with reconstitution date
- Use sterile technique for all withdrawals
- Never freeze reconstituted solutions
✓ Storage Best Practices
- Use original pharmaceutical vials
- Check expiration dates regularly
- Maintain temperature logs
- Store in dedicated laboratory refrigerator
- Keep detailed storage records
✗ Avoid These Mistakes
- Domestic refrigerators (temperature fluctuations)
- Bathroom or kitchen storage areas
- Direct sunlight or heat sources
- Frequent freeze-thaw cycles
- Storage in non-sterile containers
Reconstitution Protocol
Safety First: Always work in a clean environment with sterile technique. Contamination during reconstitution can compromise your entire research batch.
Required Equipment
Sterile Syringes
1ml syringes with 28-30 gauge needles OR 3ml syringes with 22-25 gauge needles
1ml syringes with 28-30 gauge needles OR 3ml syringes with 22-25 gauge needles
Bacteriostatic Water
0.9% Benzyl Alcohol solution (pharmaceutical grade)
0.9% Benzyl Alcohol solution (pharmaceutical grade)
Alcohol Swabs
70% isopropyl alcohol prep pads
70% isopropyl alcohol prep pads
Clean Work Surface
Dedicated laboratory area or laminar flow hood
Dedicated laboratory area or laminar flow hood
Vial Specifications: Our compounds are provided in 3ml pharmaceutical-grade vials with rubber stoppers. These vials are vacuum-sealed to preserve compound integrity during storage and shipping.
Dilution Calculator
Interactive Calculator: For precise dilution calculations, use our online Peptide Dilution Calculator - it automatically calculates the exact bacteriostatic water needed for your specific requirements.
Final Concentration = (Peptide Amount in mg) ÷ (Total Volume in ml)
Standard 3ml Vial Dilution Examples:
- 10mg vial + 1ml Bac Water = 10mg/ml concentration
- 10mg vial + 2ml Bac Water = 5mg/ml concentration
- 10mg vial + 2.5ml Bac Water = 4mg/ml concentration
- 20mg vial + 2ml Bac Water = 10mg/ml concentration
- 20mg vial + 2.5ml Bac Water = 8mg/ml concentration
3ml Vial Advantage: Our 3ml pharmaceutical vials provide ample space for reconstitution volumes up to 2.5ml, allowing for flexible concentration options while maintaining easy measurement and withdrawal.
Understanding Vacuum-Sealed Vials
Vacuum Considerations: Our 3ml pharmaceutical vials are vacuum-sealed to preserve compound integrity. This creates a partial vacuum inside the vial that will assist with bacteriostatic water injection.
✓ Vacuum Handling Tips
- Allow vacuum to assist injection but maintain control
- Use gentle back-pressure on syringe plunger
- Inject slowly (10-15 seconds for 2ml)
- Aim for vial wall, not directly onto powder
- Listen for slight "hiss" when needle penetrates
✗ Avoid These Issues
- Letting vacuum draw water too rapidly
- Fighting against the vacuum pressure
- Injecting directly onto lyophilized cake
- Using excessive force on syringe plunger
- Panicking if water draws in quickly initially
Step-by-Step Reconstitution
-
Prepare Workspace
Clean work surface with 70% alcohol. Ensure adequate lighting and remove all non-essential items from the area. -
Hand Hygiene
Wash hands thoroughly with antimicrobial soap. Consider wearing nitrile gloves for additional protection. -
Inspect Components
Check peptide vial for cracks or contamination. Verify bacteriostatic water clarity and expiration date. -
Sanitize Vial Tops
Clean both peptide and bacteriostatic water vial tops with alcohol swabs. Allow to air dry for 30 seconds. -
Draw Bacteriostatic Water
Using a sterile syringe, draw the calculated volume of bacteriostatic water. Remove air bubbles by tapping syringe. Note: Draw slightly more than needed to account for dead space in needle. -
Inject Into Peptide Vial (Vacuum Considerations)
Important: 3ml vials are typically under vacuum. Insert needle at 45-degree angle into peptide vial. The vacuum may draw bacteriostatic water in automatically - control the syringe plunger to maintain slow injection speed. Inject water slowly down the side of the vial wall to avoid aggressive foaming caused by rapid vacuum draw.If vacuum is strong, allow it to assist but maintain control by applying gentle back-pressure on the plunger to regulate flow rate. Target injection time of 10-15 seconds for 2ml volume. -
Gentle Mixing
Swirl gently to dissolve. DO NOT shake vigorously. Allow 2-3 minutes for complete dissolution. -
Visual Inspection
Solution should be clear and colorless. Any cloudiness, particles, or discoloration indicates contamination. -
Label and Date
Mark vial with reconstitution date, concentration, and batch number for tracking purposes. -
Immediate Storage
Place in refrigerator immediately after reconstitution. Do not leave at room temperature.